Marionette strain
WebSep 25, 2024 · Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. 26 November 2024. Adam J. Meyer, Thomas H. Segall-Shapiro, … Christopher A. Voigt. Download PDF. WebNov 11, 2024 · Current technologies for controlling gene expression lack systemization and fall short of this goal, a limitation that motivated the development of the “Marionette” strains in Escherichia coli, which can allow for robust and independent inducible expression of many genes within a single bacterial cell.
Marionette strain
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WebApr 23, 2024 · “Marionette” strains for prototyping 243 different expression profiles for lycopene pathway enzymes (Meyer et al., 2024), and the characterization of thousands of ribosome binding site combinations for tuning the production of limonene (Jervis et al., 2024) and naringenin (Zhou et Escherichia coli DH10B (New England BioLabs, Ipswich, MA, USA) was used for all routine cloning and directed evolution. Plasmid-based regulator systems were characterized in E. coli DH10B. Marionette-Wild, -Clo, and -Pro were derived from E. coli MG1655 (ref. 51), E. coli DH10B, and E. coli BL21 (New … See more Cells were induced with the following chemicals: 2,4-diacetylphophloroglucinol (DAPG) ≥97% purity from Santa Cruz Biotechnology (sc-206518); cuminic acid (Cuma) ≥98% purity from Millipore Sigma (268402); 3 … See more For routine transformations, strains were made competent for chemical transformation. Overnight cultures (250 μl for E. coli DH10B derived cells, 100 μl for E. coli MG1655 and BL21 derived cells) were subcultured into … See more For Supplementary Fig. 12: mid-log induction time course: glycerol stocks of strains containing the plasmids of interest were streaked on LB … See more All measurements shown were taken by cytometry of cells in mid-log growth except when noted. Glycerol stocks of strains containing the plasmids of interest were streaked on LB + … See more
WebMar 20, 2024 · These “Marionette” strains allow for the independent control of gene expression using 12 small-molecule inducers. © 2024, The Author(s), under exclusive … WebWe have taken steps towards this “Marionette” strain by constructing and testing an initial set of 4 orthogonal sensors, engineered by placing bacterial operator elements into yeast …
WebMar 20, 2024 · These “Marionette” strains allow for the independent control of gene expression using 2,4-diacetylphophloroglucinol (DAPG), cuminic acid (Cuma), 3-oxohexanoyl-homoserine lactone (OC6), vanillic... WebJan 7, 2024 · Meyer A, Segall Shapiro T, Glassey E, Zhang J, Voigt C. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2024;15:196-204 pubmed publisher product information
WebApr 23, 2024 · The resulting “Marionette” strains are able to respond 12 different small molecules, with low background expression and over 100-fold induction. Adam Meyer, the lead author of this work, describes how he envisions the applications of this system: “ Our next experiments will explore how Marionette can be applied to quickly optimize the ...
WebMay 26, 2024 · Here, we developed a multi-input transcriptional circuit to regulate the MVA pathway, where four chemical inducers, l-arabinose (Ara), choline chloride (Cho), cuminic acid (Cuma), and isopropyl β-d-1-thiogalactopyranoside (IPTG), each regulate one of four orthogonal promoters. incentive\u0027s tjWebFeb 4, 2024 · The Marionette strains could make optimizing heterologous metabolic pathways faster and easier by expanding the design space for complex genetic circuits. … income gro cash sure lifetime payoutWebMarionette strains aim to make refining metabolic pathways faster and easier The Voigt lab at The Massachusetts Institute of Technology re-cently reported the optimization of 12 … incentive\u0027s thWebApr 25, 2024 · Our strains can learn the mastery of 3×3 board games such as tic-tac-toe, even when starting from a completely ignorant state. We provide a general genetic mechanism for the autonomous learning of decisions in changeable environments. income graphic designerWebWhat were the requirements for the Marionette strains? 1. No cross talk between inducers / promoters. 2. Transferable between strains. What is the importance of the Marionette system? 1. Allows to regulate gene expression. 2. Metabolic burden control. 3. … incentive\u0027s tmWebJan 18, 2024 · Microbial marionettes Directed evolution of a set of 12 small-molecule-responsive biosensors and their integration into Escherichia coli “marionette” strains enables researchers to precisely... income generation ideas for charitiesWebA Simple Resistance - dpB.010 -Marionette strain: Abstract. Here, we characterised the expression of our Processor plasmid with the Transcriptional unit dpB.010 [PCin - mScarlet]. We showed that variation of the concentration of pCin’s inducer OCH14 increase the expression level of the construct when mesured at the plate reader ... income group 2020